The Global Partnership for the Prevention of Armed Conflict wants to see immediate action by the Papua New Guinea government to recover illegal weapons.
The call comes after the massacre of at least 16 women and children in Hela Province early this month.
The partnership's Sharon Bhagwan Rolls said urgent action is needed to ensure the protection of everyone, especially women and children.
She said the onus was on the PNG government, and agencies at the regional level, to initiate gun control and disposal measures.
"I think it is really important that we are looking at a weapons disposal programme. I think that it's vital that communities, particularly women and children are safe at all times. I think it is really, really vital that if we are talking about prevention of armed conflict or armed violence, then the first thing is weapons disposal."
She said all Pacific nations are signatories to the Boe Declaration on human security and she expected them to meet its commitments.