Flight Centre will axe a further 160 staff and close 23 more stores due to the ongoing impacts of Covid-19.
Its managing director, David Coombes, told customers in an email on Monday the cuts reflected the lack of available work for people in its stores at the moment as borders remained closed to international tourism.
"We are gutted to again be reducing our team and farewelling a large number of talented and passionate people from our business. Our people are at the core of who we are, so this is of course, heartbreaking.
"Our people were offered, and a number accepted, voluntary redundancy."
Coombes said he was confident the business would survive the pandemic.
"These decisions, however difficult, are made to do just that so we can continue to support our customers through the impact of Covid-19.
"As borders remain closed we have been forced to make this difficult decision to ensure our business stays viable, protecting our customers' refunds and credits, and then opening up the world for Kiwis as border restrictions lift."
Flight Centre was one of the companies to benefit from the government's $47.6 million travel reimbursement scheme, as almost $700m in travel expenditure was locked up overseas because trips were cancelled due to the coronavirus.
It had also received $11.1m in wage subsidies from the government.