Iran has announced it will break a limit set on uranium enrichment, in breach of the landmark 2015 deal designed to curb its nuclear ambitions.
Deputy foreign minister Abbas Araqchi said Iran still wanted to salvage the deal but blamed European countries for failing to live up to their own commitments.
The US unilaterally withdrew from the agreement in 2018. It has since reimposed tight sanctions affecting the Iranian economy.
The Iranian announcement marks the latest breach of the accord.
In May, Iran stepped up its production of enriched uranium, which can be used to make fuel for reactors but also for nuclear weapons.
The country has already stockpiled more enriched uranium than permitted under the terms of the deal.
Iran has strongly denied that it has any intention of building nuclear weapons.
On the anniversary of the US withdrawal, Iran gave a 60-day deadline to the remaining signatories of the deal - China, France, Germany, Russia and the UK - to protect it from US sanctions.
Speaking at a press conference on Sunday at the end of that deadline, Mr Araqshi said Iran would start enriching uranium above a concentration of 3.67 percent within a few hours, to provide fuel for its Bushehr power plant.
Officials previously said this would mean a concentration of about 5 percent. Weapons-grade uranium is 90 percent enriched or more.
However, Iran's Atomic Energy Organisation spokesman Behrouz Kamalvandi said that Iran would not be making fuel for its Tehran reactor, which requires a concentration of 20 percent.
"We will enrich uranium based on our needs," he said. "Right now we don't need to enrich uranium for the Tehran reactor."
Mr Araqchi said Iran would keep reducing its commitment to the 2015 deal every 60 days.
But he also stressed that diplomacy was still an option, provided the sanctions are lifted.
Israel's energy minister Yuval Steinitz has already criticised the move, saying that while the increase was "moderate", Iran had "begun its march... toward nuclear weaponry".
The announcement comes a day after French President Emmanuel Macron spoke to Iranian President Hassan Rouhani and expressed his "strong concern" about what would happen if the agreement was abandoned.
Mr Rouhani called on European countries to act now to save the deal.
What is the deal
Six countries signed the Joint Comprehensive Plan of Action in 2015 to curb Iran's nuclear programme.
Tehran agreed to let in international inspectors and to limit its nuclear activities, in exchange for an end to tight economic sanctions.
Under the deal, Iran is only permitted to produce low-enriched uranium, which has a 3-4 percent concentration of U-235, and can be used to produce fuel for nuclear power plants.
The deal also restricted Iran to stockpiling no more than 300kg (661lb) of the low-enriched uranium.
According to the Arms Control Association, a stockpile of 1,050kg could be further enriched later into enough material to build one bomb.